modified version of the vorum silver stain protocol (Vorum Research Corporation)
90
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Vorum Research Corporation
modified version of the vorum silver stain protocol
Modified Version Of The Vorum Silver Stain Protocol, supplied by Vorum Research Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/silver+staining+protocol/vorum+silver+staining/pm39254660-508-1-11
Average 90 stars, based on 1 article reviews
Modified Version Of The Vorum Silver Stain Protocol, supplied by Vorum Research Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/silver+staining+protocol/vorum+silver+staining/pm39254660-508-1-11
Average 90 stars, based on 1 article reviews
modified version of the vorum silver stain protocol - by Bioz Stars,
2026-11
90/100 stars
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Modification:Article Title: LTBP-3 promotes early metastatic events during cancer cell dissemination Article Snippet: Silver staining of gels for analysis of 293 cell CM SF-CM was collected from equal number of 293 cells expressing different expression constructs and concentrated 10X using Amicon Ultra-4 Centrifugal filter units with 10 kDa cut-off membranes (Millipore, Billerica, MA; #UFC801024). Article Title: Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis Article Snippet: The Article Title: Proteomic identification of a novel isoform of collapsin response mediator protein-2 in spinal nerves peripheral to dorsal root ganglia. Article Snippet: Primary afferent fibers are originated from pseudounipolar sensory cells in dorsal root ganglia (DRG) and transmit external stimuli received in the skin to the spinal cord.. Here we undertook a proteomic approach to uncover the polarity of primary afferent fibers.. Lumbar spinal nerve segments, peripheral and central to DRG, were dissected from 5-wk-old Wistar rats and the lysates were subjected to large-sized 2-DE at pH 5–6. Article Title: Involvement of S -nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide Article Snippet: After the Article Title: Proteome profile of salt gland-rich epidermis extracted from a salt-tolerant tree species. Article Snippet: Preparation of proteins from salt-gland-rich tissues of mangrove plant is necessary for a systematic study of proteins involved in the plant’s unique desalination mechanism.. Extraction of high-quality proteins from the leaves of mangrove tree species, however, is difficult due to the presence of high levels of endogenous phenolic compounds.. In our study, preparation of proteins from only a part of the leaf tissues (i.e. salt gland-rich epidermal layers) was required, rendering extraction even more challenging. Article Title: S100A9, GIF and AAT as potential combinatorial biomarkers in gastric cancer diagnosis and prognosis. Article Snippet: Purpose: We have mined the gastric fluid proteome for potential gastric cancer (GC) biomarkers that may enhance disease detection and facilitate prognostic monitoring.. Experimental design: In biomarker discovery, a total of 12 patient gastric fluid samples (stages I, III, IV and gastritis) were analysed by 2DE for expression changes that correlated with GC status or disease progression.. Gastric fluid proteins showing differential expression with GC were identified by MALDI-TOF/TOF MS as putative biomarkers. Article Title: Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis – a proteomic study Article Snippet: The gels were visualised by Article Title: Proteomics-Guided Study on Buyang Huanwu Decoction for Its Neuroprotective and Neurogenic Mechanisms for Transient Ischemic Stroke: Involvements of EGFR/PI3K/Akt/Bad/14-3-3 and Jak2/Stat3/Cyclin D1 Signaling Cascades. Article Snippet: Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) formula, has been used for recovering neurological dysfunctions and treating post-stroke disability in China for 200 years.. In the present study, we investigated the effects of BHD on inhibiting neuronal apoptosis, promoting proliferation and differentiation of neural stem cells (NSCs) and neurite formation and enhancing learning and memory functional recovery in an experimental rat ischemic stroke model. BHD significantly reduced infarct volume and decreased cell apoptosis in the ischemic brain.. BHD enhanced neuronal cell viability in vitro. Silver Staining:Article Title: LTBP-3 promotes early metastatic events during cancer cell dissemination Article Snippet: Silver staining of gels for analysis of 293 cell CM SF-CM was collected from equal number of 293 cells expressing different expression constructs and concentrated 10X using Amicon Ultra-4 Centrifugal filter units with 10 kDa cut-off membranes (Millipore, Billerica, MA; #UFC801024). Article Title: Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis Article Snippet: The Article Title: Proteomic identification of a novel isoform of collapsin response mediator protein-2 in spinal nerves peripheral to dorsal root ganglia. Article Snippet: Primary afferent fibers are originated from pseudounipolar sensory cells in dorsal root ganglia (DRG) and transmit external stimuli received in the skin to the spinal cord.. Here we undertook a proteomic approach to uncover the polarity of primary afferent fibers.. Lumbar spinal nerve segments, peripheral and central to DRG, were dissected from 5-wk-old Wistar rats and the lysates were subjected to large-sized 2-DE at pH 5–6. Article Title: Involvement of S -nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide Article Snippet: After the Article Title: Proteome profile of salt gland-rich epidermis extracted from a salt-tolerant tree species. Article Snippet: Preparation of proteins from salt-gland-rich tissues of mangrove plant is necessary for a systematic study of proteins involved in the plant’s unique desalination mechanism.. Extraction of high-quality proteins from the leaves of mangrove tree species, however, is difficult due to the presence of high levels of endogenous phenolic compounds.. In our study, preparation of proteins from only a part of the leaf tissues (i.e. salt gland-rich epidermal layers) was required, rendering extraction even more challenging. Article Title: S100A9, GIF and AAT as potential combinatorial biomarkers in gastric cancer diagnosis and prognosis. Article Snippet: Purpose: We have mined the gastric fluid proteome for potential gastric cancer (GC) biomarkers that may enhance disease detection and facilitate prognostic monitoring.. Experimental design: In biomarker discovery, a total of 12 patient gastric fluid samples (stages I, III, IV and gastritis) were analysed by 2DE for expression changes that correlated with GC status or disease progression.. Gastric fluid proteins showing differential expression with GC were identified by MALDI-TOF/TOF MS as putative biomarkers. Article Title: Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis – a proteomic study Article Snippet: The gels were visualised by Article Title: Proteomics-Guided Study on Buyang Huanwu Decoction for Its Neuroprotective and Neurogenic Mechanisms for Transient Ischemic Stroke: Involvements of EGFR/PI3K/Akt/Bad/14-3-3 and Jak2/Stat3/Cyclin D1 Signaling Cascades. Article Snippet: Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) formula, has been used for recovering neurological dysfunctions and treating post-stroke disability in China for 200 years.. In the present study, we investigated the effects of BHD on inhibiting neuronal apoptosis, promoting proliferation and differentiation of neural stem cells (NSCs) and neurite formation and enhancing learning and memory functional recovery in an experimental rat ischemic stroke model. BHD significantly reduced infarct volume and decreased cell apoptosis in the ischemic brain.. BHD enhanced neuronal cell viability in vitro. Staining:Article Title: LTBP-3 promotes early metastatic events during cancer cell dissemination Article Snippet: Silver staining of gels for analysis of 293 cell CM SF-CM was collected from equal number of 293 cells expressing different expression constructs and concentrated 10X using Amicon Ultra-4 Centrifugal filter units with 10 kDa cut-off membranes (Millipore, Billerica, MA; #UFC801024). Article Title: Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis Article Snippet: The Article Title: Proteomic identification of a novel isoform of collapsin response mediator protein-2 in spinal nerves peripheral to dorsal root ganglia. Article Snippet: Primary afferent fibers are originated from pseudounipolar sensory cells in dorsal root ganglia (DRG) and transmit external stimuli received in the skin to the spinal cord.. Here we undertook a proteomic approach to uncover the polarity of primary afferent fibers.. Lumbar spinal nerve segments, peripheral and central to DRG, were dissected from 5-wk-old Wistar rats and the lysates were subjected to large-sized 2-DE at pH 5–6. Article Title: Involvement of S -nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide Article Snippet: After the Article Title: Proteome profile of salt gland-rich epidermis extracted from a salt-tolerant tree species. Article Snippet: Preparation of proteins from salt-gland-rich tissues of mangrove plant is necessary for a systematic study of proteins involved in the plant’s unique desalination mechanism.. Extraction of high-quality proteins from the leaves of mangrove tree species, however, is difficult due to the presence of high levels of endogenous phenolic compounds.. In our study, preparation of proteins from only a part of the leaf tissues (i.e. salt gland-rich epidermal layers) was required, rendering extraction even more challenging. Article Title: S100A9, GIF and AAT as potential combinatorial biomarkers in gastric cancer diagnosis and prognosis. Article Snippet: Purpose: We have mined the gastric fluid proteome for potential gastric cancer (GC) biomarkers that may enhance disease detection and facilitate prognostic monitoring.. Experimental design: In biomarker discovery, a total of 12 patient gastric fluid samples (stages I, III, IV and gastritis) were analysed by 2DE for expression changes that correlated with GC status or disease progression.. Gastric fluid proteins showing differential expression with GC were identified by MALDI-TOF/TOF MS as putative biomarkers. Article Title: Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis – a proteomic study Article Snippet: The gels were visualised by Article Title: Proteomics-Guided Study on Buyang Huanwu Decoction for Its Neuroprotective and Neurogenic Mechanisms for Transient Ischemic Stroke: Involvements of EGFR/PI3K/Akt/Bad/14-3-3 and Jak2/Stat3/Cyclin D1 Signaling Cascades. Article Snippet: Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) formula, has been used for recovering neurological dysfunctions and treating post-stroke disability in China for 200 years.. In the present study, we investigated the effects of BHD on inhibiting neuronal apoptosis, promoting proliferation and differentiation of neural stem cells (NSCs) and neurite formation and enhancing learning and memory functional recovery in an experimental rat ischemic stroke model. BHD significantly reduced infarct volume and decreased cell apoptosis in the ischemic brain.. BHD enhanced neuronal cell viability in vitro. Membrane:Article Title: LTBP-3 promotes early metastatic events during cancer cell dissemination Article Snippet: Silver staining of gels for analysis of 293 cell CM SF-CM was collected from equal number of 293 cells expressing different expression constructs and concentrated 10X using Amicon Ultra-4 Centrifugal filter units with 10 kDa cut-off membranes (Millipore, Billerica, MA; #UFC801024). Article Title: Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis Article Snippet: The Article Title: Proteomic identification of a novel isoform of collapsin response mediator protein-2 in spinal nerves peripheral to dorsal root ganglia. Article Snippet: Primary afferent fibers are originated from pseudounipolar sensory cells in dorsal root ganglia (DRG) and transmit external stimuli received in the skin to the spinal cord.. Here we undertook a proteomic approach to uncover the polarity of primary afferent fibers.. Lumbar spinal nerve segments, peripheral and central to DRG, were dissected from 5-wk-old Wistar rats and the lysates were subjected to large-sized 2-DE at pH 5–6. Article Title: Involvement of S -nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide Article Snippet: After the Article Title: Proteome profile of salt gland-rich epidermis extracted from a salt-tolerant tree species. Article Snippet: Preparation of proteins from salt-gland-rich tissues of mangrove plant is necessary for a systematic study of proteins involved in the plant’s unique desalination mechanism.. Extraction of high-quality proteins from the leaves of mangrove tree species, however, is difficult due to the presence of high levels of endogenous phenolic compounds.. In our study, preparation of proteins from only a part of the leaf tissues (i.e. salt gland-rich epidermal layers) was required, rendering extraction even more challenging. Article Title: S100A9, GIF and AAT as potential combinatorial biomarkers in gastric cancer diagnosis and prognosis. Article Snippet: Purpose: We have mined the gastric fluid proteome for potential gastric cancer (GC) biomarkers that may enhance disease detection and facilitate prognostic monitoring.. Experimental design: In biomarker discovery, a total of 12 patient gastric fluid samples (stages I, III, IV and gastritis) were analysed by 2DE for expression changes that correlated with GC status or disease progression.. Gastric fluid proteins showing differential expression with GC were identified by MALDI-TOF/TOF MS as putative biomarkers. Article Title: Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis – a proteomic study Article Snippet: The gels were visualised by Article Title: Proteomics-Guided Study on Buyang Huanwu Decoction for Its Neuroprotective and Neurogenic Mechanisms for Transient Ischemic Stroke: Involvements of EGFR/PI3K/Akt/Bad/14-3-3 and Jak2/Stat3/Cyclin D1 Signaling Cascades. Article Snippet: Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) formula, has been used for recovering neurological dysfunctions and treating post-stroke disability in China for 200 years.. In the present study, we investigated the effects of BHD on inhibiting neuronal apoptosis, promoting proliferation and differentiation of neural stem cells (NSCs) and neurite formation and enhancing learning and memory functional recovery in an experimental rat ischemic stroke model. BHD significantly reduced infarct volume and decreased cell apoptosis in the ischemic brain.. BHD enhanced neuronal cell viability in vitro. Biotin Switch Assay:Article Title: LTBP-3 promotes early metastatic events during cancer cell dissemination Article Snippet: Silver staining of gels for analysis of 293 cell CM SF-CM was collected from equal number of 293 cells expressing different expression constructs and concentrated 10X using Amicon Ultra-4 Centrifugal filter units with 10 kDa cut-off membranes (Millipore, Billerica, MA; #UFC801024). Article Title: Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis Article Snippet: The Article Title: Proteomic identification of a novel isoform of collapsin response mediator protein-2 in spinal nerves peripheral to dorsal root ganglia. Article Snippet: Primary afferent fibers are originated from pseudounipolar sensory cells in dorsal root ganglia (DRG) and transmit external stimuli received in the skin to the spinal cord.. Here we undertook a proteomic approach to uncover the polarity of primary afferent fibers.. Lumbar spinal nerve segments, peripheral and central to DRG, were dissected from 5-wk-old Wistar rats and the lysates were subjected to large-sized 2-DE at pH 5–6. Article Title: Involvement of S -nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide Article Snippet: After the Article Title: Proteome profile of salt gland-rich epidermis extracted from a salt-tolerant tree species. Article Snippet: Preparation of proteins from salt-gland-rich tissues of mangrove plant is necessary for a systematic study of proteins involved in the plant’s unique desalination mechanism.. Extraction of high-quality proteins from the leaves of mangrove tree species, however, is difficult due to the presence of high levels of endogenous phenolic compounds.. In our study, preparation of proteins from only a part of the leaf tissues (i.e. salt gland-rich epidermal layers) was required, rendering extraction even more challenging. Article Title: S100A9, GIF and AAT as potential combinatorial biomarkers in gastric cancer diagnosis and prognosis. Article Snippet: Purpose: We have mined the gastric fluid proteome for potential gastric cancer (GC) biomarkers that may enhance disease detection and facilitate prognostic monitoring.. Experimental design: In biomarker discovery, a total of 12 patient gastric fluid samples (stages I, III, IV and gastritis) were analysed by 2DE for expression changes that correlated with GC status or disease progression.. Gastric fluid proteins showing differential expression with GC were identified by MALDI-TOF/TOF MS as putative biomarkers. Article Title: Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis – a proteomic study Article Snippet: The gels were visualised by Article Title: Proteomics-Guided Study on Buyang Huanwu Decoction for Its Neuroprotective and Neurogenic Mechanisms for Transient Ischemic Stroke: Involvements of EGFR/PI3K/Akt/Bad/14-3-3 and Jak2/Stat3/Cyclin D1 Signaling Cascades. Article Snippet: Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) formula, has been used for recovering neurological dysfunctions and treating post-stroke disability in China for 200 years.. In the present study, we investigated the effects of BHD on inhibiting neuronal apoptosis, promoting proliferation and differentiation of neural stem cells (NSCs) and neurite formation and enhancing learning and memory functional recovery in an experimental rat ischemic stroke model. BHD significantly reduced infarct volume and decreased cell apoptosis in the ischemic brain.. BHD enhanced neuronal cell viability in vitro. Incubation:Article Title: LTBP-3 promotes early metastatic events during cancer cell dissemination Article Snippet: Silver staining of gels for analysis of 293 cell CM SF-CM was collected from equal number of 293 cells expressing different expression constructs and concentrated 10X using Amicon Ultra-4 Centrifugal filter units with 10 kDa cut-off membranes (Millipore, Billerica, MA; #UFC801024). Article Title: Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis Article Snippet: The Article Title: Proteomic identification of a novel isoform of collapsin response mediator protein-2 in spinal nerves peripheral to dorsal root ganglia. Article Snippet: Primary afferent fibers are originated from pseudounipolar sensory cells in dorsal root ganglia (DRG) and transmit external stimuli received in the skin to the spinal cord.. Here we undertook a proteomic approach to uncover the polarity of primary afferent fibers.. Lumbar spinal nerve segments, peripheral and central to DRG, were dissected from 5-wk-old Wistar rats and the lysates were subjected to large-sized 2-DE at pH 5–6. Article Title: Involvement of S -nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide Article Snippet: After the Article Title: Proteome profile of salt gland-rich epidermis extracted from a salt-tolerant tree species. Article Snippet: Preparation of proteins from salt-gland-rich tissues of mangrove plant is necessary for a systematic study of proteins involved in the plant’s unique desalination mechanism.. Extraction of high-quality proteins from the leaves of mangrove tree species, however, is difficult due to the presence of high levels of endogenous phenolic compounds.. In our study, preparation of proteins from only a part of the leaf tissues (i.e. salt gland-rich epidermal layers) was required, rendering extraction even more challenging. Article Title: S100A9, GIF and AAT as potential combinatorial biomarkers in gastric cancer diagnosis and prognosis. Article Snippet: Purpose: We have mined the gastric fluid proteome for potential gastric cancer (GC) biomarkers that may enhance disease detection and facilitate prognostic monitoring.. Experimental design: In biomarker discovery, a total of 12 patient gastric fluid samples (stages I, III, IV and gastritis) were analysed by 2DE for expression changes that correlated with GC status or disease progression.. Gastric fluid proteins showing differential expression with GC were identified by MALDI-TOF/TOF MS as putative biomarkers. Article Title: Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis – a proteomic study Article Snippet: The gels were visualised by Article Title: Proteomics-Guided Study on Buyang Huanwu Decoction for Its Neuroprotective and Neurogenic Mechanisms for Transient Ischemic Stroke: Involvements of EGFR/PI3K/Akt/Bad/14-3-3 and Jak2/Stat3/Cyclin D1 Signaling Cascades. Article Snippet: Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) formula, has been used for recovering neurological dysfunctions and treating post-stroke disability in China for 200 years.. In the present study, we investigated the effects of BHD on inhibiting neuronal apoptosis, promoting proliferation and differentiation of neural stem cells (NSCs) and neurite formation and enhancing learning and memory functional recovery in an experimental rat ischemic stroke model. BHD significantly reduced infarct volume and decreased cell apoptosis in the ischemic brain.. BHD enhanced neuronal cell viability in vitro. Neutralization:Article Title: LTBP-3 promotes early metastatic events during cancer cell dissemination Article Snippet: Silver staining of gels for analysis of 293 cell CM SF-CM was collected from equal number of 293 cells expressing different expression constructs and concentrated 10X using Amicon Ultra-4 Centrifugal filter units with 10 kDa cut-off membranes (Millipore, Billerica, MA; #UFC801024). Article Title: Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis Article Snippet: The Article Title: Proteomic identification of a novel isoform of collapsin response mediator protein-2 in spinal nerves peripheral to dorsal root ganglia. Article Snippet: Primary afferent fibers are originated from pseudounipolar sensory cells in dorsal root ganglia (DRG) and transmit external stimuli received in the skin to the spinal cord.. Here we undertook a proteomic approach to uncover the polarity of primary afferent fibers.. Lumbar spinal nerve segments, peripheral and central to DRG, were dissected from 5-wk-old Wistar rats and the lysates were subjected to large-sized 2-DE at pH 5–6. Article Title: Involvement of S -nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide Article Snippet: After the Article Title: Proteome profile of salt gland-rich epidermis extracted from a salt-tolerant tree species. Article Snippet: Preparation of proteins from salt-gland-rich tissues of mangrove plant is necessary for a systematic study of proteins involved in the plant’s unique desalination mechanism.. Extraction of high-quality proteins from the leaves of mangrove tree species, however, is difficult due to the presence of high levels of endogenous phenolic compounds.. In our study, preparation of proteins from only a part of the leaf tissues (i.e. salt gland-rich epidermal layers) was required, rendering extraction even more challenging. Article Title: S100A9, GIF and AAT as potential combinatorial biomarkers in gastric cancer diagnosis and prognosis. Article Snippet: Purpose: We have mined the gastric fluid proteome for potential gastric cancer (GC) biomarkers that may enhance disease detection and facilitate prognostic monitoring.. Experimental design: In biomarker discovery, a total of 12 patient gastric fluid samples (stages I, III, IV and gastritis) were analysed by 2DE for expression changes that correlated with GC status or disease progression.. Gastric fluid proteins showing differential expression with GC were identified by MALDI-TOF/TOF MS as putative biomarkers. Article Title: Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis – a proteomic study Article Snippet: The gels were visualised by Article Title: Proteomics-Guided Study on Buyang Huanwu Decoction for Its Neuroprotective and Neurogenic Mechanisms for Transient Ischemic Stroke: Involvements of EGFR/PI3K/Akt/Bad/14-3-3 and Jak2/Stat3/Cyclin D1 Signaling Cascades. Article Snippet: Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) formula, has been used for recovering neurological dysfunctions and treating post-stroke disability in China for 200 years.. In the present study, we investigated the effects of BHD on inhibiting neuronal apoptosis, promoting proliferation and differentiation of neural stem cells (NSCs) and neurite formation and enhancing learning and memory functional recovery in an experimental rat ischemic stroke model. BHD significantly reduced infarct volume and decreased cell apoptosis in the ischemic brain.. BHD enhanced neuronal cell viability in vitro. Electrofocusing:Article Title: LTBP-3 promotes early metastatic events during cancer cell dissemination Article Snippet: Silver staining of gels for analysis of 293 cell CM SF-CM was collected from equal number of 293 cells expressing different expression constructs and concentrated 10X using Amicon Ultra-4 Centrifugal filter units with 10 kDa cut-off membranes (Millipore, Billerica, MA; #UFC801024). Article Title: Standard Dyes for Total Protein Staining in Gel-Based Proteomic Analysis Article Snippet: The Article Title: Proteomic identification of a novel isoform of collapsin response mediator protein-2 in spinal nerves peripheral to dorsal root ganglia. Article Snippet: Primary afferent fibers are originated from pseudounipolar sensory cells in dorsal root ganglia (DRG) and transmit external stimuli received in the skin to the spinal cord.. Here we undertook a proteomic approach to uncover the polarity of primary afferent fibers.. Lumbar spinal nerve segments, peripheral and central to DRG, were dissected from 5-wk-old Wistar rats and the lysates were subjected to large-sized 2-DE at pH 5–6. Article Title: Involvement of S -nitrosylation of actin in inhibition of neurotransmitter release by nitric oxide Article Snippet: After the Article Title: Proteome profile of salt gland-rich epidermis extracted from a salt-tolerant tree species. Article Snippet: Preparation of proteins from salt-gland-rich tissues of mangrove plant is necessary for a systematic study of proteins involved in the plant’s unique desalination mechanism.. Extraction of high-quality proteins from the leaves of mangrove tree species, however, is difficult due to the presence of high levels of endogenous phenolic compounds.. In our study, preparation of proteins from only a part of the leaf tissues (i.e. salt gland-rich epidermal layers) was required, rendering extraction even more challenging. Article Title: S100A9, GIF and AAT as potential combinatorial biomarkers in gastric cancer diagnosis and prognosis. Article Snippet: Purpose: We have mined the gastric fluid proteome for potential gastric cancer (GC) biomarkers that may enhance disease detection and facilitate prognostic monitoring.. Experimental design: In biomarker discovery, a total of 12 patient gastric fluid samples (stages I, III, IV and gastritis) were analysed by 2DE for expression changes that correlated with GC status or disease progression.. Gastric fluid proteins showing differential expression with GC were identified by MALDI-TOF/TOF MS as putative biomarkers. Article Title: Identification of differentially regulated proteins in a patient with Leber's Congenital Amaurosis – a proteomic study Article Snippet: The gels were visualised by Article Title: Proteomics-Guided Study on Buyang Huanwu Decoction for Its Neuroprotective and Neurogenic Mechanisms for Transient Ischemic Stroke: Involvements of EGFR/PI3K/Akt/Bad/14-3-3 and Jak2/Stat3/Cyclin D1 Signaling Cascades. Article Snippet: Buyang Huanwu Decoction (BHD), a classic traditional Chinese medicine (TCM) formula, has been used for recovering neurological dysfunctions and treating post-stroke disability in China for 200 years.. In the present study, we investigated the effects of BHD on inhibiting neuronal apoptosis, promoting proliferation and differentiation of neural stem cells (NSCs) and neurite formation and enhancing learning and memory functional recovery in an experimental rat ischemic stroke model. BHD significantly reduced infarct volume and decreased cell apoptosis in the ischemic brain.. BHD enhanced neuronal cell viability in vitro. |